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gram's method

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Word: Gram's Method

Part of Speech: Noun

Definition: Gram's method is a laboratory technique used to classify bacteria based on their cell wall properties. In this method, bacteria are first stained with a purple dye called gentian violet. Then, they are treated with a solution known as Gram's solution. After this, the bacteria are decolorized with alcohol and stained again with another dye called safranin. The result is that bacteria that keep the purple color are called "gram-positive," while those that lose the purple color and take up the pink color from safranin are called "gram-negative."

Usage Instructions: Gram's method is often used in microbiology to identify and differentiate between various types of bacteria. It is usually performed in a laboratory by trained professionals.

Example: "The scientist used Gram's method to determine whether the bacteria in the sample were gram-positive or gram-negative."

Advanced Usage: In advanced microbiology or clinical settings, understanding Gram's method can help in diagnosing infections and choosing appropriate antibiotics, as gram-positive and gram-negative bacteria may respond differently to treatments.

Word Variants: - Gram-positive (adjective): Refers to bacteria that retain the gentian violet stain. - Gram-negative (adjective): Refers to bacteria that do not retain the gentian violet stain and take on the safranin color.

Different Meanings: The term "Gram" in this context refers to Hans Christian Gram, the scientist who developed the staining technique. It is not used in other contexts but is specific to microbiology.

Synonyms: There are no direct synonyms for Gram's method, but it is often referred to as "Gram staining" or "Gram stain."

Idioms and Phrasal Verbs: There are no idioms or phrasal verbs directly related to Gram's method, as it is a technical term specific to microbiology.

Noun
  1. a staining technique used to classify bacteria; bacteria are stained with gentian violet and then treated with Gram's solution; after being decolorized with alcohol and treated with safranine and washed in water, those that retain the gentian violet are Gram-positive and those that do not retain it are Gram-negative

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